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Cell pellets were lysed with 800 l of buffer C (20 mM Tris-Cl (pH = 8.0), 150 mM NaCl, 1.5 mM MgCl2, 10 mM KCl, 10% Glycerol, 0.5 mM EDTA, 0.5 % NP-40, 1x protease and 1x phosphatase inhibitors)

8a (iii)), supporting a similar colonization peak

Confocal fluorescence microscopy Primary BMMs (3040% confluence) seeded onto a confocal dish were stimulated to osteoclast phenotype with 30 ng/mL M-CSF and 50 ng/mL RANKL and treated with 2.5 10 6 cells/mL SP for 30 min

C.et al (2014)

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