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For instance, composite nanofibers composed of gelatin, chitosan, collagen, or sodium alginate can be fabricated via electrospinning to create porous scaffolds that mimic the extracellular matrix, promoting cell adhesion and proliferation (e.g., spindle-shaped human dermal fibroblasts on gelatin/chitosan scaffolds) ( The fundamental attributes of hydrogels lie in their three-dimensional hydrophilic polymer networks, which exhibit biocompatibility, biodegradability, and high drug-loading capacity, enabling on-demand drug release in response to environmental stimuli (e.g., pH and temperature) ( 4.3.4 Microneedle delivery systems Microneedles represent a minimally invasive drug delivery platform composed of microscale needles (height: 102000 m

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Also theres a possibility that the needle hit a larger blood vessel which has bled into the muscle thus causing pain and stiffness

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coli plasmid pCzn1 to obtain the recombinant plasmid peSAM-FVpE