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The complete tumor stem medium (CTSM) was prepared by adding the following growth factor supplements to the TSM-basal: 50x-diluted B-27 supplement minus vitamin A (Gibco, Thermofisher Scientific, Reinach, Switzerland), Heparin solution (2 ng ml 1 , 0.2%, Stem Cell Technologies, Vancouver, BC, Canada), and human epidermal growth factor (20 ng ml 1 , H-EGF), human basic fibroblast growth factor (20 ng ml 1 , H-FGF-basic-154), human platelet-derived growth factor-aa (20 ng ml 1 , H-PDGF-AA), human platelet-derived growth factor-bb (20 ng ml 1 , H-PDGF-BB) were purchased from Shenandoah Biotechnology (Warwick, PA, USA)
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Fatima M, Almalki WH, Khan T, Sahebkar A, Kesharwani P
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Both B12 shots and IV infusions bypass the gastrointestinal tract entirely, eliminating this dose-dependent absorption loss