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To reduce background staining, the cells were quenched for 10min with 50nM glycine in TBS and blocked for 30min with 5% Normal Horse Serum (NHS) or Normal Goat Serum (NGS) in 0,2% gelatin-TBS solution, depending on the isotype of the secondary antibody

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10.1016/j.cell.2017.03.014 26 Prakash BabuN.PaolaM.Hamers AnoukA