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The gender distribution in both samples was not significantly different from an expected equal distribution, as indicated by a chi-square goodness-of-fit test (Young: X 2 = 0.783, p = 0.37, Old: X 2 = 0.08, p = 0.87

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Concordantly with the pharmacological experiments, GCLM deficiency reduced cellular tolerance to FA, which could be reverted by expressing GCLM in trans (Fig

PCR was performed in 20 l reaction mixture containing 5 ng of the obtained cDNA (or 50 ng of human genomic DNA as a control), 1x Phusion HF buffer, 0.2 mM dNTP mix, 0.5 M of each primer and 0.4 U Phusion DNA polymerase (F530S, Thermo Scientific)

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