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After neutralization with 0.5 M phosphate buffer (7.5), 0.1 mL of the supernatant was added to a reaction mixture containing 0.2 mM NADPH, 100 mM phosphate buffer (pH 7.5), 5 mM EDTA, and 0.6 mM 5,5dithio-bis (2-nitrobenzoic acid)

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the proportion of positively stained cells was divided into four grades, namely (1) 025%, (2) 2650%, (3) 5175%, and (4) 76100%

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Circular RNAs are miRNA sponges and can be used as a new class of biomarker