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The plate was incubated for 2 min at room temperature on a shaker at 300 r.p.m

Fusion primers with dual-indexed PCR adapters were used to generate the construct library, and short fragments were removed with AMPure beads

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Chemical warfare agents: chemistry, pharmacology, toxicology, and therapeutics

doi: 10.1016/j.immuni.2024.03.021 85 KempSBCarpenterESSteeleNGDonahueKLNwosuZCPachecoAet al

More specifically, cellular replication of the cytochrome C Bax protein triggers the activation of the caspase-3 protease, which is the primary molecular mechanism of apoptosis (220)