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Baek M, DiMaio F, Anishchenko I, Dauparas J, Ovchinnikov S, Lee GR, et al

(2014) Comparative effects of adding -mercaptoethanol or L-ascorbic acid to culture or vitrification-warming media on IVF porcine embryos

doi: 10.1016/j.phymed.2016.02.009, 56

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As such, you may not be able to obtain 4 full doses of 0.25 mL from each vial