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Kramann (RWTH Aachen), was cultured in Dulbeccos Modified Eagle Medium F-12 Nutrient Mixture with GlutaMAX (DMEM/F12 GlutaMAX, Thermo Fisher, 10565018) supplemented with 10% (v/v) FBS (Thermo Fisher, 41966029), 100 U/ml penicillin, and 100 g/ml streptomycin (Pen/Strep, Thermo Fisher, 15140122)

doi: 10.1093/humrep/17.4.921

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When compared to healthy individuals, a few studies have demonstrated that there were increased levels of C-reactive proteins, inflammatory mediators such as IL-6 and IL-8 and inflammatory cytokines in IBS patients (Matricon et al., 2012

Your body also doesn't store vitamin C, so consistent daily intake matters more than large doses