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When designing a KLOW 80mg protocol, researchers calculate the working concentration from their chosen reconstitution volume and then determine per-use volumes from there

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Peaks corresponding to the monoisotopic M mass of the peptide itself (2347.175) were seen in control and treated samples, while a peak corresponding to the M mass of the peptide plus the mass of the 4-OXO-DHA (341.21) of 2688.385 was only seen in the 4-OXO-DHA treated peptide sample