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The cells were then stimulated with 0.5 mM H 2 O 2 for 24 h, and the number of cells was determined with a Cell Counting Kit-8 (Dojindo) according to the manufacturers protocol

Moreover, therapeutic strategies targeting the gut-brain axis, including interventions aimed at restoring gut microbial composition, metabolite balance, and reducing inflammation, could be beneficial in mitigating CKD progression associated with chronic stress and mental health issues

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Inhibition of phosphodiesterase: A novel therapeutic target for the treatment of mild cognitive impairment and Alzheimers disease