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The ovary, Malpighian tubules, salivary gland and midguts of the engorged females were dissected in phosphate buffer, frozen with liquid nitrogen and stored at -80 C, and three different developmental periods, including eggs, larvae and adults, were collected for subsequent assays

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Cell culture and seeding for experiments NIH-3T3, U937 and neuroblastoma cells, SH-SY5Y, were obtained from ATCC and were maintained in HyQ-DMEM media with 5% Fetal Clone III, (Gibco/BRL), 50 U/ml penicillin, and 100 g/ml streptomycin in 100 mm Petri dishes

Decreased glutathione accelerates neurological deficit and mitochondrial pathology in familial ALS-linked hSOD1(G93A) mice model

Gastroenterology 152 , 787798.e2 (2017)

10.1002/ana.21600 [DOI] [PMC free article] [PubMed] [Google Scholar] Ursini F., Maiorino M