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doi:10.1101/cshperspect.a009183 Acknowledgments This work was supported by the Chang Gung Memorial Hospital [Grants CMRPD1A0531, CMRPD1C0231, CMRPG391311, CMRPG3A1131] and the Ministry of Science and Technology, Taiwan [Grant NMRPD181001-3]

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Product Facts Systane ULTRA is a sterile solution containing polyethylene Glycol 400, Propylene Glycol, Hydroxypropyl Guar, Sorbitol, Aminomethylpropanol, Boric acid, Potassium Chloride, Sodium Chloride, and POLYQUAD (Polyquaternium-1) 0.001% as preservative

After 14 days of incubation, colonies were stained using INT (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride) to visualize viable cells