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RT-PCR and sequencing analysis of viral RNA Plaque purified viruses were used to infect HeLa cells and at 7 hr postinfection, total cytoplasmic RNAs were extracted with 1 ml Trizol reagent (Invitrogen)

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The conclusion of whether the system regenerates ATP cannot be clear cut because reaction 8, another positive control, is only giving 0.43% yield (calculated from theoretical product titre predicted from the model)

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This results in enhanced production of pro-inflammatory cytokines, including TNF-, IL-6, and IL-1 (Rofaeil et al

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