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Let everything reach room temperature first

Low abundance is effectively enriched by this removal

(1987) Production of superoxide anions by a CNS macrophage, the microglia

To assess if the metabolism of TNBS by NAPDH-linked enzymes in aerobic solution results in formation of ROS, we determined the formation of hydrogen peroxide in an aqueous solution (PBS) containing 0.25 mm NADPH, TNBS (1 mm) and GSSG reductase (15 U/ml) over time

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Bocian-Ostrzycka KM, asica AM, Dunin-Horkawicz S, Grzeszczuk MJ, Drabik K, Dobosz AM, Godlewska R, Nowak E, Collet J-F, Jagusztyn-Krynicka EK (2015) Functional and evolutionary analyses of Helicobacter pylori HP0231 (DsbK) protein with strong oxidative and chaperone activity characterized by a highly diverged dimerization domain