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Microscopy From rats, at end of the experiment, the brain, liver, kidney, stomach, duodenum, jejunum, colon, rectum, lungs, and heart were fixed in 10% neutral buffered formalin (pH 7.4) at room temperature for 24 h

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[63] Other early medical investigators made similar observations of EDTA's role in the treatment of cardiovascular disease (Bechtel, 1956