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For immunofluorescence, tumor cells grown on poly-lysine-treated coverslides were fixed with 4% paraformaldehyde for 15 min at RT and permeabilized with cold methanol (20 C) for 5 min or with 0.1% Triton X-100/PBS at RT for 15 min before incubated overnight at 4 C with primary antibodies ( Supplementary Table 3 )

Clioquinol, a halogenated 8-hydroxyquinoline derivative, has been found to regulate iron homeostasis in tissues by effectively chelating Fe 2+ , thereby inhibiting ferroptosis (227)

Following all protocols optimizes chances of achieving upper range results

Sulforaphane attenuation of type 2 diabetes-induced aortic damage was associated with the upregulation of Nrf2 expression and function

Drinking a lot of alcohol or taking certain medicines can also affect B12 absorption

Wichakorn Jitphaphun, M.D