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For instance, vitamin A (an in vivo product derived mainly from -carotene) is an excellent scavenger of singlet oxygen, blocking and/or reverting lipid peroxidation in the plasma membrane (Sies and Stahl, Reduction of the Cochlear Blood Flow and Vasodilators Exposure to loud noise, in addition to inducing oxidative stress, also diminishes cochlear blood flow by reducing blood vessel diameter and erythrocyte velocity in the basilar membrane, the spiral ligament and the SV (Sendowski, 2+ ) which seem to have protective effects in the noise-exposed cochlea (Figure 2)

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with a few exceptions, no statistically significant differences were shown, primarily due to methodological issues stemming from the significant variability of nocturnal melatonin production and 6-hydroxymelatonin excretion, as well as the timing of their age-related decline (Wu and Swaab 2005)

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RNA integrity was verified by 1% agarose gel electrophoresis, and RNA concentration and purity were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA)