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Furthermore, mTORC1 is also a transcriptional regulator of autophagy since it inactivates some transcription factors that are involved in lysosomal biogenesis and function and that sustain autophagy activity 28

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Importantly, we selected hNPCs for our transcriptomic analysis rather than cortical spheroids, as they represent a homogenous cell population for the identification of genetic changes associated with neurodevelopment

An equal volume of normal saline was added to dilute the blood, followed by centrifugation at 3,000 rpm for 10 minutes