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The XFe96 FluxPak (Agilent, 102416-100) cartridge was hydrated in water overnight before calibrating with Seahorse XF Calibrant Solution (Agilent, 103681-100)

doi: 10.1210/er.2008-0027 3 OlarescuNCGunawardaneKHansenTKMollerNJorgensenJOL

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DSIP stands for Delta Sleep-Inducing Peptide a reference to its proposed role in promoting delta wave sleep, the deepest and most physically restorative stage of the sleep cycle