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J., Van Der Wal, G., & Ferrier, M
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Elevated blood concentration of methylmalonic acid is a specific clinical indicator of vitamin B 12 deficiency
Methylcobalamin is the bioactive form of B12 and does not require conversion
It was not until a genetically engineered strain of Pseudomonas denitrificans was used, in which eight of the genes involved in the biosynthesis of the vitamin had been overexpressed, that the complete sequence of methylation and other steps could be determined, thus fully establishing all the intermediates in the pathway