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The pellet was dissolved in 0.01 M Tris-HCl buffer (pH 7.8) and a second round of ultracentrifugation was performed as before but without a sucrose cushion

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Like, give him space to breathe, try to talk a little slower at him, because hes going to react better to you because hes hes a very kinesthetic guy

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This involved a direct search for the telomere sequence 5-CCCTAAA-3 and its reverse complement