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Wakefulness time measurement To evaluate the impact of Pichia pastoris secreted recombinant peptides on insomnia, we analyzed the increase in wakefulness time among different groups of mice: Control, Model, GABA, DSIP-CBBBP, DSIP, and CBBBP
High performance liquid chromatography (HPLC) The detection and quantification of dopamine was performed in a similar protocol as previously described [26]
doi: 10.4172/2155-9880.1000201
[3] While human trials remain limited, these findings raise interest in possible therapeutic applications
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