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With age, it decreases naturally in the body, along with bringing on premature aging of the skin, pigmentation issues, and a rather fading complexion

The remaining fat particles were digested with 0.2% collagenase (Gibco, Billings, MT, USA) in a water bath shaker at 37 C for 30 min, and the mixture was centrifuged at 1000 rpm for 5 min to remove undigested fat

Given their high plasticity and stem-cell-like characteristics, reactive astrocytes have been investigated as potential therapeutic targets for in neurological disorders such as ischemic stroke

Tissues were counterstained with haematoxylin, mounted with Permount mounting medium (Fisher Scientific, SP15500) and coverslipped for imaging