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At each time point, 1/10 volume of culture medium per well CCK-8 solution reagent was added, incubated at 37C for Frontiers in Bioengineering and Biotechnology | www.frontiersin.org 2April 2021 | Volume 9 | Article 677576 fbioe-09-677576 April 24, 2021 Time: 18:16 # 3 Huang et al