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Proteins were transferred onto a NC or PVDF membrane (Fisher Scientific) and blocked with 5% milk for 1 h

Fekete T, Mszros M, Szegletes Z, Vizsnyiczai G, Zimnyi L, Deli MA, et al

Since this SSE is associated with a loss of sensitivity and accuracy, the above-mentioned sample preparation methods describe compromises between the minimization of these matrix effects by removing matrix components during sample clean-up, and the ability to detect a broad spectrum of analytes with reasonable sample preparation efforts and adequate sensitivity

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