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These e-cigs function by using a small, heated coil (atomizer) to vaporize a cartridge of liquid solution (usually containing a decided concentration of nicotine) into an aerosol mist

Schiller D, Monfils M-H, Raio CM, Johnson DC, LeDoux JE, Phelps EA

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Anxiety-like behavior in rodents is often assessed using open field and elevated plus maze tests, in which more time spent in the periphery of open field or in the closed arms of the elevated plus maze are associated with anxiety-like effects (76)