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The cells were subsequently transfected with Lipofectamine RNAiMax (13778150, Invitrogen, USA) to introduce siRNAs targeting various genes (Table S1)

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Standard Research Protocol Dose: 300 mcg per day subcutaneous injection Timing: Morning, fasted state (at least 30 minutes before food or exercise for maximal absorption) Cycle length: Typically 8 to 12 weeks on, followed by a 4-week rest period Reconstitution: Standard BAC water reconstitution