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Cells were extensively washed with PBS, fixed in 4% PFA for 15 min, permeabilized and blocked with 0.01% saponin/10% normal goat/PBS serum and stained with the primary antibodies in the same blocking solution in a humid chamber

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The pre-mixed format makes reconstitution simple and supports efficient dosing in in vitro assays, cell culture, or animal injury models

In 2003, the famous Ralph Carmel wrote in the New England Journal of Medicine: The administration of folic acid can mask the megaloblastic anaemia caused by cobalamin deficiency

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