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Acetyl-L-carnitine supplementation to old rats partially reverts the age-related mitochondrial decay of soleus muscle by activating peroxisome proliferator-activated receptor gamma coactivator-1alpha-dependent mitochondrial biogenesis

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Contact with the round window membrane is maintained for 30 minutes by placing the animal in a recumbent position Intratympanic injectionEach animal is positioned so that the head is tilted at an angle to favor injection towards the round window niche

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This allows it to directly neutralize reactive oxygen species (ROS) and reactive nitrogen species (RNS), such as hydroxyl radicals, superoxide anions, and peroxynitrite