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Sequencing was performed using a HiSeq 2500 (Illumina), with a read length of 300 bp paired-end reads and at least 25,000 tags per sample

Gene expression analysis To obtain further insight into the mechanisms of BPC-157 participation in cell signal transduction, we carried out a quantitative real-time PCR using a Signal Transduction Path-wayFinder RT2 Profiler PCR Array, which contained a set of primers for 84 key genes representative of 18 different signal transduction pathways

The protective effects of serum on normal cells are moderate but do not interfere with the hyperthermic reduction of leukemic cells

However, maintaining a nutritious diet can synergize with the treatment

Relaxin and its receptors in prostate cancer show evidence for associations between relaxin signaling and progression

By combining them, researchers can investigate a broader range of biological mechanisms while maintaining consistency across experimental designs