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To avoid this: Always do a patch test before full use

Liu et al., 2017

However, since no efforts are made to explain where the alleged apoptotic cells are located and the TUNEL assay always shows TUNEL-positive cells in the area of necrosis [56], it appears that these parameters are mainly being used to increase the amount of data in the manuscript rather than as a convincing mechanistic argument for apoptotic cell death [57]

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