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Eyes were exposed to 15% followed by 30% sucrose gradient and embedded in OCT media and frozen

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Goodrick JC, Xu M, Finnegan R, Schilling BM, Schiavi S, Hoppe H, Wan NC (2001) High-level expression and stabilization of recombinant human chitinase produced in a continuous constitutive Pichia pastoris expression system

Results showed that ethanolic extract had no effect on the duration of mice immobility while the aqueous extract significantly diminished it, concluding that aqueous extract of silymarin has antidepressant effect in animal models (53)

Holbrook KA, Byers PH, Counts DF, Hegreberg GA