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Masson's staining was performed using a Masson's trichrome staining kit in accordance with the manufacturer's protocol to observe collagen deposition at room temperature for a total of 15 min (Wiegert's iron hematoxylin, 8 min

The results showed that inhibiting HDAC5 significantly increased CD8 + T cell infiltration in the tumor microenvironment, reduced the proportion of Tregs, and exhibited synergistic effects with anti-PD-1 antibodies, jointly inhibiting tumor growth (69)

In a rat model, injection of berberine and atorvastatin resulted in a decrease in ET-1 plasma levels

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