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After incubation, absorption at 450 nm was measured and translated to the cell viability with a standard curve

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Moreover, GHK was also reported to decrease the gene expression of IGF-1 (Pickart et al., 2014), which stimulates TGF-1 transcription and protein expression in dermal fibroblasts in vitro (Ghahary et al., 1998)

HUBERMAN: While I'm not opposed to that approach, you just want to make sure that the dosing, or I should say the relative dosing of each peptide is such that, you know, You're avoiding unnecessary increases in prolactin and cortisol, and that you're not hitting a pathway redundantly