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For example, genetic KO mice may suffer from developmental compensation, whereas pharmacological reagents may have non-specific effects, both of which may complicate the interpretation of the data

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Also, key enzymes that consume NAD+ called sirtuins, PARPs, and NAD+ glycohydrolases have distinct roles in aging and age-related diseases

Nature 489(7415):318321 Mattison JA, Colman RJ, Beasley TM, Allison DB, Kemnitz JW, Roth GS, Ingram DK, Weindruch R, De Cabo R, Anderson RM (2017) Caloric restriction improves health and survival of rhesus monkeys

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