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The membrane was washed three times with TTBS for 5 min each and incubated with horseradish peroxidase conjugated goat anti-mouse IgG (Sigma) diluted (1:3000) in TTBS at 37C for 1 h

Higher flow rates will decrease production time, but may result in losing a small portion of the target protein

Six-week cycles have been extensively studied in research literature and provide a well-characterized timeframe for many research uses