50 mM Tris pH 8.0 150 mM NaCl 1 mM DTT 0.5 mM EDTA Temperature: 4C or room temperature Ratio: 1:100 (TEV:substrate by OD280) Failure 4: Incomplete Cleavage (You Get a Mixture) The problem: Even when cleavage works, it's rarely 100%
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