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The ERK1/2 mitogen-activated protein kinase pathway as a master regulator of the G1- to S-phase transition

50 L of supernatant was mixed with 450 L of 40 mM Tris-HCl buffer and 10 L of 10 M 2,7-dichlorofluorescein diacetate and incubated at 37C for 30 min in the dark

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Mater 33 (7), 2006892

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However, supplementation has become a popular method for boosting levels