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Although in those early studies, HDX was analyzed for insulin, -lactoglobulin, myoglobin, and poly-DL-alanine, because of the use of densitometry as analytical technique, the application of this method was limited by poor spatial resolution (early densitometric analysis measured average deuterium incorporation over large segments of a protein, which made it impossible to pinpoint specific sites of exchange)

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Khavinson, I.G