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Lentivirus production and infection First, 293T cells were cotransfected with lentiviral plasmids along with packaging plasmids psPAX2 (Addgene, 12260) and pMD2.G (Addgene, 12259) using polyethylenimine transfection reagent (Polysciences, 9002-98-6)

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pH profiles indicative of rate-limiting nucleophilic displacement in thioltransferase catalysis

Furthermore, the analysis pipeline of the NGS data has been modified and improved from a manual to a semi-automated process

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