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Alfhili MA, Alamri HS, Alsughayyir J, Basudan AM

reported an increase in the protein-bound form of glutathione in human blood after oral administration, while glutathione in the deproteinized fraction did not change [12]

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The reactions were allowed to incubate in the dark at 37 C for 30 min, during which fluorescence was measured in a SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices, San Jose, CA, USA) using excitation in the range of 530560 nm and emission detection at 590 nm

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