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When a drug inhibits or activates an enzyme, substrates accumulate and products deplete generating a characteristic perturbation signature detectable by mass spectrometry

Think of it as the cofactor that makes your cellular engine run

17 = 5,65

The constitutive active sites are 1, 2, and 5 that are responsible for caspase-like (C-like), trypsin-like (T-like), and chymotryptic activity (CT-like), respectively

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