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A more comprehensive approach would be to concurrently measure actual protein levels (e.g., WB, ELISA, etc.) of potential targets in addition to inhibitor assays

2019 Sep 3;11(9):2073

doi: 10.1016/j.ymgme.2007.01.010 142 LevensonC

Confocal laser scanning microscopy (CLSM) analysis Seeds of two transgenic lines expressing redox-sensitive GFP2 (roGFP2) either in the cytosol (Grx1-roGFP2) or the plastids (TKTP-Grx1-roGFP2) 92 and Col-0 WT seedlings were sown onto 6-well cell culture plates (Sigma-Aldrich, CLS3516) containing 3 ml of liquid MS medium (pH 5.7) supplemented with 2% sucrose

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It has been shown that rhein inhibits CYP2C9, CYP1A2, CYP2E1, CYP2D6 and CYP3A enzymes in rat liver (Tang et al., 2009)