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The next step after this would be the validation of the identified hub proteins by conducting further in vitro as well as in vivo studies
Boschetto P, Miotto D, Bononi I, Faggian D, Plebani M, Papi A, Creminon C, De Rosa E, Fabbri LM, Mapp CE
We observed no effect of this handling on the survival or growth of wildtype cells, but gspD mutants were highly dependent on copper in the media, confirming that the cells need to express GspD in order to survive and grow (Fig
Intracellular ROS production Intracellular reactive oxygen species (ROS) levels were measured in treated cells using the oxidant-sensitive probe 2,7dichlorofluorescein diacetate [H 2 DCFDA] 11
It is whether the protocol around it, the cycle structure, the rest period, the sequencing with other compounds, and the biological fit with the patient, is designed correctly
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