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Quick math for common doses at 2.5 mg/mL: 0.25 mg: 0.25 / 2.5 x 100 = 10 units 0.50 mg: 0.50 / 2.5 x 100 = 20 units 1.0 mg: 1.0 / 2.5 x 100 = 40 units 1.7 mg: 1.7 / 2.5 x 100 = 68 units 2.4 mg: 2.4 / 2.5 x 100 = 96 units If you reconstituted with 3.0 mL instead (1.67 mg/mL), the same doses become 15, 30, 60, 102, and 144 units respectively

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Inhibition of renal prostaglandin synthesis can lead to a variety of detrimental effects if kidney function is less than optimal, including fluid and electrolyte disorders, acute renal dysfunction, nephrotic syndrome, interstitial nephritis, and renal papillary necrosis

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Media was replaced every 2 days and monolayer integrity measured by TEER ( cm 2 , Millicell-ERS Voltohmmeter, Merck Millipore) every 7 days (16 h after media change)