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Next, we employed a fluorescence polarization assay (FPA) to pinpoint the sequences crucial for binding among the specified regions, subsequently improving the cell applicability of the peptide sequence

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All nanobody incubations were performed in BrainPhys medium (StemCell Technologies) to enhance synaptic activity and the diluted nanobodies were centrifuged in a tabletop centrifuge at maximum speed for 10 min at room temperature

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