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Further systematic research is required to establish a definitive relationship among these three components

Materials and methods Primary human hepatocyte isolation Primary human hepatocytes (PHH) were prepared from nontumorous tissues obtained from freshly resected human livers of different donors (PHH Ctrl, n = 4) or explanted livers of WD patients (PHH WD, n = 2) via a two-step perfusion protocol as previously described [8]

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This increases production of the stress hormone cortisol which disrupts the normal hair growth cycle

Rather than treating these domains independently, we propose a unifying framework in which melatonin functions as a physiological hub coordinating temporal organization and cellular protection

The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm