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Neutralization of HIV and simian immunodeficiency virus with short peptide tags introduced into the Env molecules at varying positions has also been described for the corresponding anti-tag antibodies 35,44,45 , which is consistent with neutralization through general steric obstruction rather than specific targeting of functional sites

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Subsequently, samples were concentrated to dryness in vacuum before dissolving in 150 L of 80% methanol (precooled at -20C), then centrifuged again at 13 500 g and 4C for 10 min to obtain the supernatant for high-performance liquid chromatography Ultimate 3000 (Thermo Fisher Scientific, USA)-tandem mass spectrometry Q Exactive Focus (Thermo Fisher Scientific, USA) (LC-MS/MS) analysis (95, 96)

showed that less than 1% of semaglutide-induced BW reduction was mediated by GI AE 107