ROS inactivate protein kinases that phosphorylate microtubule-associated proteins (MAPs) that maintain this dynamic equilibrium (65)
Glutathione is a natural antioxidants present in our body, produced by the liver
For one-step reactions, all components were mixed in 96-well microtitre plates in 100 l volume (PS, F-Bottom, clear, Greiner) and reactions were performed for 30 min in a Spark 20 M (Tecan) plate reader at 30 C
Cell Culture and GSH Preparation K562, HepG2, Hela and L-02 cells were respectively cultured in RPMI 1640 (Hyclone, penicillin 100 U/mL, streptomycin 100 g/mL) adding 10% fetal bovine serum and maintained at 37 C in a humidified atmosphere including 5% CO 2 , in terms of the instructions of the American Type Culture Collection
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